Charles J Frazier

Charles J Frazier, Ph.D.

Associate Professor, Graduate Coordinator

Department: Pharmacodynamics
Business Phone: (352) 273-7686
Business Email: cjfraz@ufl.edu

Research Profile

My lab specializes in cellular neurophysiology and optical imaging. In a broad sense, the goal of our work is to contribute to the development of a thorough mechanistic understanding of how excitability is regulated, and information is processed, at the synaptic level in the mammalian CNS. We also attempt to use such information to develop a better understanding of how the brain fails to work in a number of abnormal or diseased states (including mood and anxiety disorders, geriatric memory dysfunction, and drug abuse). We believe that the type of basic mechanistic information that our work seeks to provide will ultimately be essential in order to develop maximally effective therapeutic interventions for such disorders.

At present we have three specific areas of research focus. The first is on centrally acting oxytocin and its role in mental health disorders, the second is on age related changes in cellular and synaptic physiology, with an emphasis on NMDA receptor and/or GABAB receptor mediated signalling in hippocampus and prefrontal cortex, and the third is on endocannabinoid mediated signalling and its ability to modulate tonic inhibition in the dentate gyrus.

In addition, we are also very interested in technology development and application in neurobiology. Consistent with that interest, we have recently completed construction of a 2-photon based laser scanning epifluorescence microscope that is well integrated into a system capable of high end conventional cellular neurophysiology. We are currently using this technology to study age related changes in calcium influx into single dendritic spines of CA1 pyramidal cells, and to evaluate the effect of autocrine activation of oxytocin receptors on centrally projecting axons of OTR positive magnocellular neurons.

Finally, we are also invested in software development for use in neurobiology and currently are developing or maintaining separate software programs designed for off-line analysis of electrophysiological data (Ophys), telemetry data (Telem-a-Gator), and line scan data from quantitative two photon imaging experiments (Scan-a-Gator).

We are often looking for highly motivated students and postdocs with a strong technical background and a sincere interest in the work. Please send inquiries about available positions directly to frazier@cop.ufl.edu.

Publications

2020
Endogenous oxytocin inhibits hypothalamic corticotrophin-releasing hormone neurones following acute hypernatraemia.
Journal of neuroendocrinology. 32(3) [DOI] 10.1111/jne.12839. [PMID] 32133707.
2020
Region-specific effects of HIV-1 Tat on intrinsic electrophysiological properties of pyramidal neurons in mouse prefrontal cortex and hippocampus.
Journal of neurophysiology. 123(4):1332-1341 [DOI] 10.1152/jn.00029.2020. [PMID] 32101482.
2019
Oxytocin Receptors Are Expressed by Glutamatergic Prefrontal Cortical Neurons That Selectively Modulate Social Recognition.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 39(17):3249-3263 [DOI] 10.1523/JNEUROSCI.2944-18.2019. [PMID] 30804095.
2019
Optogenetic dissection of basolateral amygdala contributions to intertemporal choice in young and aged rats.
eLife. 8 [DOI] 10.7554/eLife.46174. [PMID] 31017572.
2017
Chronic salt-loading reduces basal excitatory input to CRH neurons in the paraventricular nucleus and accelerates recovery from restraint stress in male mice.
Physiology & behavior. 176:189-194 [DOI] 10.1016/j.physbeh.2017.03.038. [PMID] 28351560.
2017
Optogenetic Inhibition Reveals Distinct Roles for Basolateral Amygdala Activity at Discrete Time Points During Risky Decision Making
The Journal of Neuroscience. 37(48):11537-11548 [DOI] 10.1523/JNEUROSCI.2344-17.2017.
2016
Age-related changes in tonic activation of presynaptic versus extrasynaptic γ-amniobutyric acid type B receptors in rat medial prefrontal cortex.
Neurobiology of aging. 45:88-97 [DOI] 10.1016/j.neurobiolaging.2016.05.015. [PMID] 27459929.
2016
Alcohol consumption increases basal extracellular glutamate in the nucleus accumbens core of Sprague-Dawley rats without increasing spontaneous glutamate release.
The European journal of neuroscience. 44(2):1896-905 [DOI] 10.1111/ejn.13284. [PMID] 27207718.
2016
Increasing brain angiotensin converting enzyme 2 activity decreases anxiety-like behavior in male mice by activating central Mas receptors.
Neuropharmacology. 105:114-123 [DOI] 10.1016/j.neuropharm.2015.12.026. [PMID] 26767952.
2016
Oxytocin depolarizes fast-spiking hilar interneurons and induces GABA release onto mossy cells of the rat dentate gyrus.
Hippocampus. 26(9):1124-39 [DOI] 10.1002/hipo.22595. [PMID] 27068005.
2016
NR2A-Containing NMDARs in the Prefrontal Cortex Are Required for Working Memory and Associated with Age-Related Cognitive Decline.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 36(50):12537-12548 [PMID] 27807032.
View on: PubMed
2015
Hydration and beyond: neuropeptides as mediators of hydromineral balance, anxiety and stress-responsiveness.
Frontiers in systems neuroscience. 9 [DOI] 10.3389/fnsys.2015.00046. [PMID] 25873866.
2015
Molecular aspects of age-related cognitive decline: the role of GABA signaling.
Trends in molecular medicine. 21(7):450-60 [DOI] 10.1016/j.molmed.2015.05.002. [PMID] 26070271.
2014
A method for high purity intestinal epithelial cell culture from adult human and murine tissues for the investigation of innate immune function.
Journal of immunological methods. 414:20-31 [DOI] 10.1016/j.jim.2014.08.002. [PMID] 25193428.
2014
Prefrontal cortical GABAergic dysfunction contributes to age-related working memory impairment.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 34(10):3457-66 [DOI] 10.1523/JNEUROSCI.5192-13.2014. [PMID] 24599447.
2013
Marijuana, endocannabinoids, and epilepsy: potential and challenges for improved therapeutic intervention.
Experimental neurology. 244:43-50 [DOI] 10.1016/j.expneurol.2011.11.047. [PMID] 22178327.
2013
Acute hypernatremia exerts an inhibitory oxytocinergic tone that is associated with anxiolytic mood in male rats.
Endocrinology. 154(7):2457-67 [DOI] 10.1210/en.2013-1049. [PMID] 23653461.
2013
Angiotensin type 1a receptors in the paraventricular nucleus of the hypothalamus protect against diet-induced obesity.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 33(11):4825-33 [DOI] 10.1523/JNEUROSCI.3806-12.2013. [PMID] 23486953.
2013
Preformed vs. on-demand: molecular economics of endocannabinoid signalling.
The Journal of physiology. 591(19):4683-4 [DOI] 10.1113/jphysiol.2013.262717. [PMID] 24085490.
2011
Development and characterization of immobilized cannabinoid receptor (CB1/CB2) open tubular column for on-line screening.
Analytical biochemistry. 412(1):85-91 [DOI] 10.1016/j.ab.2010.12.034. [PMID] 21215722.
2011
Key questions of endocannabinoid signalling in the CNS: which, where and when?
The Journal of physiology. 589(Pt 20):4807-8 [DOI] 10.1113/jphysiol.2011.219493. [PMID] 22001725.
2011
Cannabinoid receptor agonists potentiate action potential-independent release of GABA in the dentate gyrus through a CB1 receptor-independent mechanism.
The Journal of physiology. 589(Pt 15):3801-21 [DOI] 10.1113/jphysiol.2011.211482. [PMID] 21646412.
2010
mGluR-mediated and endocannabinoid-dependent long-term depression in the hilar region of the rat dentate gyrus.
Neuropharmacology. 58(4-5):712-21 [DOI] 10.1016/j.neuropharm.2009.12.022. [PMID] 20045707.
2010
Muscarinic receptor activation modulates the excitability of hilar mossy cells through the induction of an afterdepolarization.
Brain research. 1318:42-51 [DOI] 10.1016/j.brainres.2010.01.011. [PMID] 20079344.
2010
Two distinct and activity-dependent mechanisms contribute to autoreceptor-mediated inhibition of GABAergic afferents to hilar mossy cells.
The Journal of physiology. 588(Pt 15):2801-22 [DOI] 10.1113/jphysiol.2009.184648. [PMID] 20547680.
2008
Excitatory afferents to CA3 pyramidal cells display differential sensitivity to CB1 dependent inhibition of synaptic transmission.
Neuropharmacology. 55(7):1140-6 [DOI] 10.1016/j.neuropharm.2008.07.007. [PMID] 18675282.
2007
Endocannabinoids in the dentate gyrus.
Progress in brain research. 163:319-37 [PMID] 17765727.
View on: PubMed
2007
Presynaptic inhibition of excitatory afferents to hilar mossy cells.
Journal of neurophysiology. 97(6):4036-47 [PMID] 17442771.
View on: PubMed
2006
Endocannabinoid-mediated depolarization-induced suppression of inhibition in hilar mossy cells of the rat dentate gyrus.
Journal of neurophysiology. 96(5):2501-12 [PMID] 16807350.
View on: PubMed
2005
Septal innervation regulates the function of alpha7 nicotinic receptors in CA1 hippocampal interneurons.
Experimental neurology. 195(2):342-52 [PMID] 16000197.
View on: PubMed
2005
Medial septal/diagonal band cells express multiple functional nicotinic receptor subtypes that are correlated with firing frequency.
Neuroscience letters. 389(3):163-8 [PMID] 16112453.
View on: PubMed
2003
Nicotinic receptors on local circuit neurons in dentate gyrus: a potential role in regulation of granule cell excitability.
Journal of neurophysiology. 89(6):3018-28 [PMID] 12611982.
View on: PubMed
1998
Acetylcholine activates an alpha-bungarotoxin-sensitive nicotinic current in rat hippocampal interneurons, but not pyramidal cells.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 18(4):1187-95 [PMID] 9454829.
View on: PubMed
1998
Synaptic potentials mediated via alpha-bungarotoxin-sensitive nicotinic acetylcholine receptors in rat hippocampal interneurons.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 18(20):8228-35 [PMID] 9763468.
View on: PubMed
1997
Elevation of basal protein kinase C activity increases ethanol sensitivity of GABA(A) receptors in rat hippocampal CA1 pyramidal neurons.
Journal of neurochemistry. 68(5):1949-59 [PMID] 9109521.
View on: PubMed
1996
Cholinergic deafferentation enhances rat hippocampal pyramidal neuron responsiveness to locally applied nicotine.
Brain research. 727(1-2):217-20 [PMID] 8842401.
View on: PubMed
1996
Platelet-derived growth factor induces a long-term inhibition of N-methyl-D-aspartate receptor function.
The Journal of biological chemistry. 271(27):16151-9 [PMID] 8663218.
View on: PubMed

Grants

Jul 2020 ACTIVE
Consequence and mechanism of diet-driven vagal remodeling on gut-brain feeding behavior
Role:
Funding: UNIV OF GEORGIA via NATL INST OF HLTH NIDDK
Jun 2019 ACTIVE
Somatostatin blockade of CNS autonomic hyperactivity for treatment of diabetic retinopathy
Role: Principal Investigator
Funding: UNIV OF ALABAMA BIRMINGHAM via NATL INST OF HLTH NEI
Jan 2019 ACTIVE
Interrogating distinct angiotensin type-1 and type-2 receptor containing brain circuits to understand and alleviate hypertension
Role:
Funding: NATL INST OF HLTH NHLBI
Sep 2018 ACTIVE
Decision making and basolateral amygdala dysfunction in aging
Role: Project Manager
Funding: NATL INST OF HLTH NIA
Dec 2017 ACTIVE
Neurons expressing angiotensin type 2 receptors in the NTS as an access point for cardiovascular control.
Role:
Funding: NATL INST OF HLTH NHLBI
Feb 2015 ACTIVE
Novel Aspects of Central Oxytocin Signaling Relevant to Mood/Anxiety Disorders
Role: Principal Investigator
Funding: NATL INST OF HLTH NIMH

Teaching Profile

Courses Taught
2016,2018-2020,2020
PHA5878C Pt Care 3: Cv and Pulm
2015-2018,2020
PHA5560 Pathphys-Pt Assess I
2009-2013,2015-2020,2020
PHA6936 Advanced Topics in Pharmaceutical Sciences
2007,2009-2012,2012-2020
PHA7980 Research for Doctoral Dissertation
2011-2012,2014-2020,2019-2020
PHA6521C Research Techniques in Pharmacodynamics
2006,2009-2020
PHA7979 Advanced Research
2007,2009-2020
PHA6512L Experiential Research Training in Pharmacodynamics
2010-2012,2015-2020,2019-2020
PHA6910 Supervised Research
2010-2012,2020
PHA6971 Research for Master’s Thesis
2019
PHA6935 Selected Topics in Pharmacy
2019
PHA5789C Patient Care 7
2018
GMS7980 Research for Doctoral Dissertation
2018
BME7980 Research for Doctoral Dissertation
2009-2010,2012-2018
PHA6938 Research Seminar
2016
GMS7794 Neuroscience Seminar
2014-2016
PHA5517 Pharmacol Bas Ther 2
2016
PHA4911 Undergraduate Research in Pharmacodynamics
2013,2015
PHA6509 Systems Physiology and Pathophysiology II
2015
PHA5561C Physiol Bas Disease 2
2007,2009-2013,2013-2014
PHA7939 Journal Colloquy in Pharmacodynamics
2014
PHA6508 Systems Physiology and Pathophysiology I
2010-2014
PHA5560C Physiol Bas Disease 1
2012
PHA5516 Basis of Therapeu 1
2010,2012
PHA5902 Research Pharmacodyna
2010
PHA4902 Prob Pharmacodynamics
2010
PHA6540 Neurochem Founda
2006-2008
GMS6022 Prin Neuroscience 2

Contact Details

Phones:
Business:
(352) 273-7686
Emails:
Business:
cjfraz@ufl.edu